Yaa Medical Cloth Injure Tending (Part-2)

MEDI-TEX WOUND CARE (PART-2)

¹Deepalakshmi.K,¹deepatextile29@gmail.com,
Textile Technology,
Jaya Engineering College, Chennai, India.
²Rajan.S,²srajusri123@gmail.com, Textile Chemistry,
SSM College of Engineering, Namakkal, India.

Part-1

RESULTS AND DISCUSSION

FT-IR Test Result

Fig 5: FTIR Spectra

The to a higher house FTIR spectra clearly shows that the leaves of mimosa pudica possess got the functional compounds similar flavonoids, steroids, glycosides, saponins, proteins, carbohydrates, alkaloids together with phenolic compounds. Among all the compounds the flavonoids found to possess got frequency attain from 1600 to 3300, so it has broad attain of frequency which has multifariousness of biological activities, the foliage extract contains highest total of flavonoids together with phenolic compounds thus influencing the lawsuit of injure healing together with antibacterial activity.

The other compounds which likewise influence the belongings of antibacterial lawsuit which likewise ranges inward the desired frequency. The tabular array below shows the frequency attain of mimosa functional compounds.

S.NO	Range	Functional Group
1	1558-1667	Alkaloids
2	1200-920	Glycosides
3	2800	Carbohydrates
4	470-490	Proteins
5	895	Steroids
6	1600-3300	Flavonoids
7	2900	Phenols

Table I: FTIR Frequency Range Values

SEM Test Result
It tin live used to examine micro textile together with crystallographic orientation inward many materials.

Fig 6: SEM of Medicated Herbal Bandage coated with Mimosa Pudica extract

The to a higher house figures demonstrate the morphological construction of the herbal treated cotton fiber gauze where the particles are settled depth into the fibre together with the fibres are oriented together with crystalline region. The herb particles are good deposited on the textile surface. Such deposit of herbal extract on the surface of the fibre was found to a greater extent than inward mimosa pudica extract treated fabric. The below figures shows the images of untreated together with treated fabric. The differences tin live clearly seen from the 2 images that the molecular orientation behavior is proficient inward treated textile than the untreated one. The particles are good deposited on the surface of the fabric.

Fig vii a:Untreated gauze

Fig vii b: Treated gauze

Absorbency Test Result (Wicking Height)
Influenza A virus subtype H5N1 good absorbent textile behaves similar a wick together with H2O rises through it against gravity. If the H2O is tinted with dye, the tiptop of the H2O bird wicked through the textile strip tin live noted. Wicking tiptop is higher for a proficient absorbent fabric. So the below tabular array shows the wicking behavior of both treated together with untreated sample.

SAMPLES	UNTREATED	TREATED
1	15	18
2	14	16.5
3	15.5	18.5
4	14.5	16
5	15	16.5
Average	14.8 (cm)	17.5 (cm)

Table II: Absorbency Test Values

The to a higher house seek is carried for xv mints for each sample.

For 1 mint the absorbency of treated sample is 8cm

For v mints the absorbency of treated sample is 12cm

For 10 mints the absorbency of treated sample is 14cm

The to a higher house results demonstrate that the treated sample has high absorbency than the untreated sample. Thus the wicking behavior is high for treated sample. Tests were likewise carried out for 1 min, v min, 10 mints , the absorbency charge per unit of measurement decreasing due to the gravity because at the higher wicking tiptop the influence of gravity is higher, hence lowering the absorption of water.

Phytochemical Test Result
Phytochemical screening of the flora extract was carried to discover the presence of phyto compounds introduce inward the extract. To position the phytochemicals inward flora extract chemic tests were carried out.

Test For Saponins
To 1 ml of extract v ml of distilled H2O was added together with shaken vigorously. Observed for foam appearance indicates the presence of saponins.

Fig 8: Foam indicates Saponins

Test for Flavonoids
To 1 ml of extract v ml of dilute ammonia solution was added, followed past times add-on of concentrated sulphuric acid along the sides of the tube. Appearance of yellowish colouration.

Fig 9: Yellow indicates Flavonoids

Test for Alkaloids
1 ml of sample was taken to that few drops of Dragandoff reagent was added together with observed for orangish crimson colour.

Fig 10: Red Orange Indicates Alkaloids

Test for Steroids
1 ml of the filtrate was taken to that 10% concentration H2SO4 was added together with observed for greenish colour.

Fig 11: Green Indicates Steroids

Anti-Bacterial Test Result (Well Diffusion Method) AATCC 147
The sample of cotton fiber gauze is inoculated inward the civilization plates containing bacteria of staphylococcus aureus together with kiebsiella pneumonia. Based on the bacterial growth on food agar plates later 24 hours of incubation with the seek specimen, the sample cotton fiber gauze is found to possess got antibacterial activity equally at that spot was a zone of inhibition against the seek organisms. As per standard, the textile stuff volition live considered to possess got antibacterial activity only when at that spot is no bacterial growth straight nether the sample inward the contact area.

Fig 12: Zone of Inhibition

Sample	Bacteriostatic activity inward mm		Growth nether fabric
	Staphylococcus   Aureus  AATCC 6538	Klebsiella Pneumonia AATCC 4352
Cotton Gauze	13mm	17mm	Nil

Table III: Antibacterial Test Result- Zone of Inhibition

Test Summary:
Technique used : Direct inoculation, Well Diffusion Method
Amount of sample taken: 5*2.5 cm for each bacterium
Incubation Period : 37 bird Celsius for 24 – 48 hours

WOUND HEALING TEST REPORT
The Mimosa Pudica ethanol extract shows real proficient injure healing activity. The ethanol herbal extract exhibited proficient injure healing activity in all probability due to the presence of phenols constituents.

Fig 13: Albino Rat

 Fig 14: Wound Inflicted on Rat

Fig 15: Medicated Herbal Bandage

Fig 16: Wound Contraction inward v Wound days

Assessment of Wound Contraction
Wound contraction was monitored past times metric mensuration of the injure area. This was studied past times tracing the raw injure expanse on a transparent polythene newspaper together with the traced expanse was measured past times using a graph paper. The injure contraction was measured equally a per centum decrease of master injure size of 500mm2 for each animate beingness of a group.

Results were expressed equally hateful ± SEM. The information were analysed past times using 1 means analysis of variance (ANOVA) followed past times Dunnet’s test. P values<0.05 were considered equally significant.

Groups	Drug Treatment	Wound Contraction(days)
		1	4	7
I	Control(Non                   Medicated Gauze)	16.17 ±0.70	35.67 ±0.34	87.07 ±1.67
II	Test Control (Medicated Gauze)	16.88 ±0.70	167.56 ±1.67***	234.98 ±12.98***

Table IV: Effect of Medicated Herbal Bandage on Wound Contraction

Values are inward Mean ± SEM; (n=6), *P<0.05, **P<0.01, ***P<0.001 Vs. Control.

Wound Healing Activity on Human

Fig 17: Wound on human ankle

  Fig 18:Herbal Bandage tied on the Wound

 Fig 19: Cured injure later 2 days

CONCLUSION
This inquiry piece of job has given a novel thought inward finishing of cotton fiber gauze with herbs of mimosa pudica for antibacterial together with injure healing activity.

The to a higher house FT-IR together with SEM analysis reveals that the herbal extract of mimosa pudica has high antibacterial together with injure healing compounds when compared to untreated samples. So it promotes ameliorate proficient healing together with reduces the opportunity of bacterial infection. Since mimosa pudica flora are rich inward flavonoids together with phenols together with abundant availability inward many of the countries the reach of implementation together with commercialization of herbal extract to impart finishes inward textile is volition live high together with raw stuff is 100% from natural resources, it is eco-friendly having economic, social together with environmental benefits. The treated fabrics were found to live real hygienic with no bacterial infection when compared to conventionally finished fabrics. In the introduce study, Mimosa Pudica leaves extract possesses a rigid injure healing activity against shrewd together with chronic wounds.

Also past times testing on Rat (Invitro study) it was found that at that spot was a pregnant growth inward the injure healing activity past times this medicated herbal bandage. Treatment of herbal bandage showing reduced days of epithelialization menstruum together with to a greater extent than contraction.

From the to a higher house findings, it could live concluded that the traditional plants may stand upwards for novel sources of injure healing with stable, biologically active components that tin constitute a scientific base of operations for the utilization of plants inward modern medicine. This report has opened upwards doors for production of ameliorate eco-friendly medically treated Band-Aid.

REFERENCES

1. Ajay V. Singh, AnisurRahman, N.V.G. Sudhir Kumar, A.S. Aditi, M. Galluzzi, S. Bovio, S. Barozzi, E. Montani, D. Parazzoli “ Bio-inspired approaches to pattern smart fabrics” – Article inward Press.
2. Ambikapathy .V, Gomathi.S, Pannerselvam. A., “ Effect of antifungal activity of about medicinal plants against Pythiumdebaryanum”- Asian Journal of flora scientific discipline together with Research, 2011, 1(3): 131-134
3. Baby Joseph, Jency George, Jeevitha Mohan “ Pharmocology together with Traditional Uses of Mimosa Pudica”- International Journal of Pharmaceutical Sciences together with Drug Research 2013; 5(2); 41-44
4. Banupriya.J, Maheshwari.V. “ Comparative report on Antibacterial Finishes past times Herbal together with Conventional Methods on the Woven Fabrics”- Journal of Textile Science together with Engineering 2013 Vol.3
5. Banupriya.J, Maheshwari.V, “ Effects of Aromal Finish past times Herbal together with Conventional Methods of Woven Fabrics”- Journal of Textile Science together with Engineering 2013 Vol. iii
6. DebasisBhunia together with Amal Kumar Mondal, “ Systematic analysis of an Aquatic Medical Plant H2O Mimosa inward Eastern India”- International magazine of Life Sciences biotechnology together with Pharma Research 2012 vol.1
7. Doss.A, Vijayasanthi.M, Parivuguna.V, Anand.S.P, “ Evaluation of Antibacterial Properties of Ethanol together with Flavonoids From Mimosa PudicaLinn.andPanicum Maximum Jacq”- Plant Sciences Feed 2011-1(2): 39-44
8. GangaiAbirami.S.K, SudhaMani.K, NishaDevi.M, NirmalaDevi.P, “ The Antimicrobial Activity of Mimosa Pudica”- International Journal of Ayurveda together with Pharma Research 2012;2(1):105-108
9. Gulab Khan Rohela, KiranSaini, Surekha together with Christopher.T, “ Screening of secondary Metabolites together with Antimicrobial activity of Mimosa Pudica”- Research Journal Of Pharmaceutical, Biological together with Chemical Sciences 2011 vol.2 474-479
10. Gulzaralam, ManjulPratap Singh, Anita singh, “ Wound Healing Potential of about medical plants”- International Journal of Pharmaceutical Sciences Review together with Research 2011 vol.9 136-145
11. Hasabo Muhammad Ahmed, Rajendran.R, Balakumar.C, “ Nanoherbal Coating of cotton fiber textile to nurture antimicrobial durability”- Applied Chemistry 2012
12. Ibrahim.D, Muhammad.I, Kanoma.A.I, Shehu.K, Aliero.A.A, Aliyu.R.U, “ Antifungal screening of Mimosa Pudica flora extracts against phytopathogenic fungi”- Open Science of Journal of Bioscience together with Bioengineering 2014; 1(1): 1-12
13. Joby Jose, SudheeshSudhakaran, Sumesh Kumar, Sony Jayaraman, JayadeviVariyar, “ Influenza A virus subtype H5N1 Comparative Evaluation of Anticancer Activities of flaconoids Isolated from Mimosa Pudica, Aloe Vera together with PhyllanthusNiruri Against Human Breast Carcinoma Cell Line using MTT Assay” – International Journal of Pharmacy together with Pharmaceutical Sciences 2014 vol.6
14. Krishna Veni, Amsa Mani, “ Preparation together with Characterization of Medical Herb GlycyrrhizaGlabra together with a report of the Anti-Microbial together with Thermal Properties on Cotton” – Journal of Textile together with Apparel, Technology together with Management 2012 vol.7
15. LubnaAzmi, Manish Kumar Singh together with Ali Kamal Akhtar, “ Pharmacological together with Biological Overview on Mimosa Pudica Linn”- International Journal of Pharmacy together with Life Sciences 2011 2(11): 1226-1234
16. Mahesh.S, Manjunatha.A.H, VijayaKumar.G, “ Studies on Antimicrobial Textile Finish using Certain Plant Natural Products” – International Conference on Advance inward Biotechnology together with Pharmaceutical Sciences 2011
17. Malone.M, “ Wound-induced hydraulic signals together with Stimulus transmission inward Mimosa Pudica”- Horiculture Research International, 1994: 49-56
18. Manish Pal Singh, Bharghava.S, Bhaduaria.R.S, Sharma.C.S, “ Wound Healing Potential of Alcoholic Extract of Mimosa Pudica Linn. Leaves”- Pharmacologyonline 2010 ;2: 32-38
19. MilindPande, AnupamPathak, “ Preliminary Pharmacognostic Evaluations together with Phytochemical Studies on Roots of Mimosa Pudica” – International Journal of Pharmaceutical Sciences Review together with Research 2010; vol.1
20. M L Gulrajini, Deepti Gupta, “ Emerging Techniques for Functional Finishingn of Textiles”- Indian Journal Of Fibre together with Textile Research 2011; vol.36, 388-397
21. M P Sathinarayanan, Bhat.N.V, Kokate.S.S, Walnuj.V.E, “ Antibacterial Finish for cotton fiber textile from herbal products” – Indian Journal of Fiber together with Textile Research 2010; vol.35: 50-58
22. Naveen Alasyam, Mannala Sunil, Jayashree.T, VinayKumar.K, Nagesh together with NarapoguVenkatanarayana, “ Evaluation of Anticonvulsant activity of alcoholic extract of Mimosa Pudica inward Swiss albino rats”- Journal of Chemical together with Pharmaceutical Research 2014 , 6(4): 1175-1179
23. Nilesh Kumar, PalwinderKaur, Kuntal Das, SudiptaChakroborty, “Mimosa Pudica L. Influenza A virus subtype H5N1 Sentitive Plant” – International Journal of Pharmacy together with Pharmaceutical Sciences, 2009. Vol.1
24. PalwinderKaur, Nilesh Kumar, Shivananda.T.N, GagandeepKaur, “ Phytochemical Screening together with antimicrobial activity of the flora extracts of Mimosa Pudica L. against selected microbes”- Journal Of Medical Plants Research, 2011, vol.5(22)
25. Parikshit.A, Champanerkar, Vikas.V, Vaidya, SunithaShailajan, Sasikumar.N, Menon, “ Influenza A virus subtype H5N1 sensitive, rapid together with validated liquid chromatography-tandem majority spectrometry method for decision of Mimosine inward Mimosa Pudica”- Natural Science 2010, vol.2; 713-717
26. Pham Ngoc Anh Thu, PhanThanh Long together with Dong ThiAnh Dao, “ Study on Extraction of Mimosine From Sensitive Plant for Drinks Production’- Food Technology
27. RajeshwariSaraswat together with RaghunathPokharkar, “ GCMS Studies of Mimosa Pudica”- International Journal of Pharm Tech Research 2012; vol.4, 93-98
28. RekhaRajendran, S.Hemalatha, K. Akasakalai, Madhukrishna, BhavanSohil, Vittal together with R. MeenakshiSundaram, “ Hepatoprotective activity of Mimosa Pudica leaves against Carbontetrachloride induced toxicity”- Journal of Natural Products, 2009; vol.2 : 116-122
29. Ramya.S, Aluguchamy.N, Maruthappan.VM, Sivaperumal.R, Sivalingam.M, Krishnan.A, Govindaraju.V, Kannan.K, JayaKumararaj.R, “ Wound Healing Ethnomedicinal Plants Popular with the Malayali Tribes inward Vattal Hills, Dharmapuri, TN, India”- 2009; 1257-1271
30. Renu Singh, Yogesh, Jasrai.T, “ Mimosa hamata, a flora with anti-pathogenic properties”- Science Research publisher 2012; vol.2 

 Part-1

Share this post